期刊
BRAIN
卷 145, 期 6, 页码 1992-2007出版社
OXFORD UNIV PRESS
DOI: 10.1093/brain/awab432
关键词
cerebral small vessel disease; endothelial cells; GWAS; TRIM47; whole-exome association study
资金
- Australia, National Health and Medical Research Council
- Canada, Canadian Institutes of Health Research
- France, French National Research Agency
- Germany, Federal Ministry of Education and Research
- Netherlands, The Netherlands Organization for Health Research and Development
- United Kingdom, Medical Research Council
- European Union [643417, 667375, 754517]
- European Research Council (ERC) under the European Union [640643]
- French National Research Agency (ANR) [ANR-14-CE12-60016, ANR-18-RHUS-0002]
- Fondation Claude Pompidou
- CHARGE infrastructure grant [R01HL105756]
- National Institute on Aging [AG033193, AG049505, AG052409, AG059421]
- NIHR Senior Investigator Award
- UK Dementia Research Institute
- NIHR Imperial College Healthcare Trust Biomedical Research Centre
- Austria, Federal Ministry of Science, Research and Economy
- European Research Council (ERC) [640643] Funding Source: European Research Council (ERC)
- Agence Nationale de la Recherche (ANR) [ANR-18-RHUS-0002] Funding Source: Agence Nationale de la Recherche (ANR)
Cerebral small vessel disease is a leading cause of stroke and cognitive decline. A comprehensive gene study identified multiple loci and genes associated with the disease. Mendelian randomization supported the causal relationship between disease severity and increased risk of stroke and Alzheimer's disease. Further functional evaluation suggested a potential role of TRIM47 in the pathophysiology of cerebral small vessel disease.
Cerebral small vessel disease is a leading cause of stroke and a major contributor to cognitive decline and dementia, but our understanding of specific genes underlying the cause of sporadic cerebral small vessel disease is limited. We report a genome-wide association study and a whole-exome association study on a composite extreme phenotype of cerebral small vessel disease derived from its most common MRI features: white matter hyperintensities and lacunes. Seventeen population-based cohorts of older persons with MRI measurements and genome-wide genotyping (n = 41 326), whole-exome sequencing (n = 15 965), or exome chip (n = 5249) data contributed 13 776 and 7079 extreme small vessel disease samples for the genome-wide association study and whole-exome association study, respectively. The genome-wide association study identified significant association of common variants in 11 loci with extreme small vessel disease, of which the chr12q24.11 locus was not previously reported to be associated with any MRI marker of cerebral small vessel disease. The whole-exome association study identified significant associations of extreme small vessel disease with common variants in the 5 ' UTR region of EFEMP1 (chr2p16.1) and one probably damaging common missense variant in TRIM47 (chr17q25.1). Mendelian randomization supports the causal association of extensive small vessel disease severity with increased risk of stroke and Alzheimer's disease. Combined evidence from summary-based Mendelian randomization studies and profiling of human loss-of-function allele carriers showed an inverse relation between TRIM47 expression in the brain and blood vessels and extensive small vessel disease severity. We observed significant enrichment of Trim47 in isolated brain vessel preparations compared to total brain fraction in mice, in line with the literature showing Trim47 enrichment in brain endothelial cells at single cell level. Functional evaluation of TRIM47 by small interfering RNAs-mediated knockdown in human brain endothelial cells showed increased endothelial permeability, an important hallmark of cerebral small vessel disease pathology. Overall, our comprehensive gene-mapping study and preliminary functional evaluation suggests a putative role of TRIM47 in the pathophysiology of cerebral small vessel disease, making it an important candidate for extensive in vivo explorations and future translational work.
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