期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 119, 期 7, 页码 1965-1979出版社
WILEY
DOI: 10.1002/bit.28100
关键词
biomimetic stimulations; bone tissue engineering; multimodal bioreactor; reconfigurability
资金
- Istituto Italiano di Tecnologia within the CRUI-CARE Agreement
Traditional bone tissue engineering has used various types of bioreactors to study osteogenic differentiation, but single-mode bioreactors often fail to mimic physiological conditions effectively. This study developed a multi-modal perfusion bioreactor that induced differentiation of SaOS-2 bone-derived cells and achieved a faster bone matrix deposition rate.
In the past decades, bone tissue engineering developed and exploited many typologies of bioreactors, which, besides providing proper culture conditions, aimed at integrating those bio-physical stimulations that cells experience in vivo, to promote osteogenic differentiation. Nevertheless, the highly challenging combination and deployment of many stimulation systems into a single bioreactor led to the generation of several unimodal bioreactors, investigating one or at mostly two of the required biophysical stimuli. These systems miss the physiological mimicry of bone cells environment, and often produced contrasting results, thus making the knowledge of bone mechanotransduction fragmented and often inconsistent. To overcome this issue, in this study we developed a perfusion and electroactive-vibrational reconfigurable stimulation bioreactor to investigate the differentiation of SaOS-2 bone-derived cells, hosting a piezoelectric nanocomposite membrane as cell culture substrate. This multimodal perfusion bioreactor is designed based on a numerical (finite dement) model aimed at assessing the possibility to induce membrane nano-scaled vibrations (with similar to 12 nm amplitude at a frequency of 939 kHz) during perfusion (featuring 1.46 dyn cm(-2) wall shear stress), large enough for inducing a physiologically-relevant electric output (in the order of 10 mV on average) on the membrane surface. This study explored the effects of different stimuli individually, enabling to switch on one stimulation at a time, and then to combine them to induce a faster bone matrix deposition rate. Biological results demonstrate that the multimodal configuration is the most effective in inducing SaOS-2 cell differentiation, leading to 20-fold higher collagen deposition compared to static cultures, and to 1.6- and 1.2-fold higher deposition than the perfused- or vibrated-only cultures. These promising results can provide tissue engineering scientists with a comprehensive and biomimetic stimulation platform for a better understanding of mechanotransduction phenomena beyond cells differentiation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据