期刊
BIOSENSORS & BIOELECTRONICS
卷 199, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113874
关键词
Nanostructured; Microbiosensor; Nanoporous gold; Platinum nanoparticles; Glucose oxidase; Lactate oxidase
类别
资金
- Fundacao de Amparo anbsp
- Pesquisa do Estado de Sao Paulo (FAPESP) [2020/11722-0]
- Consejo Nacional de Inves-tigaciones Cientificas y Tecnicas (CONICET)
- European Regional Development Fund (ERDF) through the COMPETE 2020 - Operational Programme for Competitiveness and Internationalization and Portuguese national funds via FCT - Fundacao para a Ciencia e Tecnologia [POCI-01-0145-FEDER-028261, CNC UIDB/04539/2020, CEMMPRE UIDB/00285/2020]
Highly sensitive and selective nanostructured lactate and glucose microbiosensors were developed for simultaneous determination in rat brain. The microbiosensors, based on carbon fiber microelectrodes modified with nanoporous gold, exhibited enhanced sensitivity and electrocatalytic properties towards H2O2 detection. The selective measurements were achieved by enzyme immobilization, permselective layer, and a null sensor. The microbiosensor platform was used to determine lactate and glucose in blood serum samples, as well as in vivo measurements of basal extracellular concentrations in different rat brain structures.
Highly sensitive and selective nanostructured lactate and glucose microbiosensors for their in vivo simultaneous determination in rat brain were developed based on carbon fiber microelectrodes (CFM) modified with nanoporous gold (NPG) using the Dynamic Hydrogen Bubble Template (DHBT) method. Electrodeposition of platinum nanoparticles (PtNP) onto the NPG film enhances the sensitivity and the electrocatalytic properties towards H2O2 detection. The nanostructured microelectrode platform was modified by glucose oxidase (GOx) and lactate oxidase (LOx) enzyme immobilization. High selective measurements were achieved by covering with a permselective layer of electropolymerized m-phenylenediamine, deposition of a Nafion (R) film and by using a null sensor. The morphological characteristics and electroanalytical performance of the microbiosensors were assessed, by scanning electron microscopy and electrochemical techniques, respectively. The PtNP/NPG/CFM shows a high sensitivity to H2O2 (5.96 A M-1 cm-2) at 0.36 V vs. Ag/AgCl, with a linear range from 0.2 to 200 mu M, and an LOD of 10 nM. The microbiosensors were applied to the simultaneous determination of lactate and glucose in blood serum samples. Moreover, the basal extracellular concentrations of lactate and glucose were measured in vivo in four different rat brain structures. These results support the potential of the microbiosensor to be used as a valuable tool to investigate brain neurochemicals in vivo.
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