期刊
BIOSENSORS & BIOELECTRONICS
卷 205, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2022.114095
关键词
Electrochemical microfluidic sensor; Graphene fiber microelectrode; Porous nickel-cobalt phosphide nanosheet ar-rays; Bioassay
类别
资金
- National Key Research and Development Program of China [2018YFA0703200]
- National Natural Science Foundation of China [21874051, 51504168]
- Science, Technology and Innovation Commission of Shenzhen Municipality [GJHZ20200731095001004]
- Program for HUST Academic Frontier Youth Team [2019QYTD11]
This study developed an integrated electrochemical microfluidic sensing platform using freestanding graphene fiber microelectrode modified with nickel-cobalt phosphide nanosheet arrays, which shows excellent sensing performances towards neurotransmitter dopamine and can be used for sensitive analysis in human serum and urine samples.
The development of high-efficient biosensing systems for rapid and sensitive detection of disease-related biomarkers in human samples is of great significance for disease diagnosis and treatment in clinical practice. In this work, we develop an integrated electrochemical microfluidic sensing platform based on freestanding graphene fiber (GF) microelectrode for bioassay. In order to improve the electrocatalytic activity of GF microelectrode, it has been modified by unique 3D well-ordered hierarchically porous nickel-cobalt phosphide (NiCoP) nanosheet arrays (NSAs). Benefiting from the excellent electrochemical properties and structural merits, the resultant NiCoP-NSAs modified GF microelectrode shows excellent sensing performances towards neurotransmitter dopamine (DA), with a high sensitivity of 5.56 mu A cm(-2) mu M-1, a low detection limit of 14 nM, as well as good selectivity, reproducibility and stability. Furthermore, in virtue of the miniaturized size and good mechanical properties, the nanohybrid GF microelectrode can be embedded into a home-made microfluidic chip to construct an integrated electrochemical microfluidic sensing device, which has been used for sensitive analysis of DA in minimal volume of human serum and urine samples, and in situ tracking DA released from neuroblastoma cells SHSY-5Y under the stimulation for physio-pathological and pharmacological study of nervous system-related diseases.
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