期刊
BIOSENSORS & BIOELECTRONICS
卷 199, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113906
关键词
Portable electrochemical biosensor; Pesticide detection; Laser-induced graphene; MnO2 nanosheets
类别
资金
- National Natural Science Foundation of China [21775083, 22076090]
- Special Founda-tion for Distinguished Taishan Scholar of Shandong Province [ZR2020ZD37]
- Shandong Provincial Natural Science Foundation
- Shandong Province Higher Educational Program for Young Innovation Talents
Developing portable, quantitative, and user-friendly analytical tools for sensitive pesticide assay is crucial for ensuring food safety. In this study, a novel electrochemical biosensor was developed for rapid detection of organophosphorus pesticide residues. The biosensor exhibited excellent analytical performance and was useful for evaluating pesticide levels in vegetables.
Developing portable, quantitative, and user-friendly analytical tools for sensitive pesticide assay is of significant importance for guaranteeing food safety. Herein, a novel electrochemical biosensor was constructed by inte-grating laser-induced graphene (LIG) electrode on polyimide (PI) foil and MnO2 nanosheets loaded on the paper for point-of-care test (POCT) of organophosphorus (OPs) residues. The principle of this biosensor relied on acetylcholinesterase (AChE)-catalyzed hydrolytic product-triggered disintegration of MnO2 nanosheets for releasing assistant DNA to initiate nicking enzyme-aided recycling amplification. In the presence of OPs, the activity of AChE was inhibited and could not initiate the cleavage of the electroactive molecules-labeled hairpin probe on the electrode, resulting in the maintenance of the electrochemical response to realize a sign-on determination of OPs. The proposed biosensor exhibited satisfactory analytical performance for OPs assay with a linear range from 3 to 4000 ng/mL and a limit of detection down to 1.2 ng/mL. Moreover, the biosensor was useful for evaluating the residual level of pesticides in the vegetables. Therefore, this novel biosensor holds great promise for OPs assay and opens a new avenue on the development of higher-performance POCT device for sensing applications in the environment and food safety fields.
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