期刊
BIOSENSORS & BIOELECTRONICS
卷 199, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113865
关键词
SARS-CoV-2 detection; CRISPR-Cas12a; Instrument-free molecular diagnostics; Self-contained microfluidic chip; Hand warmer
类别
资金
- National Institutes of Health, United States [R01 EB023607, R61 AI154642]
In this study, a simple, sensitive, and instrument-free diagnostic method for SARS-CoV-2 was developed using a self-contained microfluidic system. The system integrates amplification, CRISPR cleavage, and lateral flow detection, allowing contamination-free visual detection. The method showed excellent sensitivity, specificity, and accuracy when clinically validated with COVID-19 samples.
Rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for early diagnostics and timely medical treatment of coronavirus disease 2019 (COVID-19). However, current detection methods typically rely on expensive and bulky instrumentation. Here, we developed a simple, sensitive, instrument-free, CRISPR-based diagnostics of SARS-CoV-2 using a self-contained microfluidic system. The microfluidic chip integrates isothermal amplification, CRISPR cleavage, and lateral flow detection in a single, closed microfluidic platform, enabling contamination-free, visual detection. To simplify the operation and transportation of the device, we lyophilized the CRISPR reagents in the reaction chamber and pre-stored the liquid solutions in blisters. We employed a low-cost, portable hand warmer to incubate the microfluidic chip without the need for electricity. The self-contained microfluidic system can detect down to 100 copies of SARS-CoV-2 RNA. Further, we clinically validated our method by detecting 24 COVID-19 clinical nasopharyngeal swab samples, achieving excellent sensitivity (94.1%), specificity (100%), and accuracy (95.8%). This simple, sensitive, and affordable microfluidic system represents a promising tool for point-of-care diagnostics of COVID-19 and other infectious diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据