期刊
BIOSENSORS & BIOELECTRONICS
卷 200, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113908
关键词
Chemical receptor; Graphene; Cadaverine; Putrescine; Gas sensor; Real-time monitoring; Portable biosensors
类别
资金
- Technology Innovation Program - Ministry of Trade, Industry & Energy (MOTIE, Korea) [20012362]
- Smart Farm Innovation Technology Development Program [421020-03]
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Ministry of Science ICT [NRF-2021M3A9I5021439]
- Research Program to Solve Urgent Safety Issues of the National Research Foundation of Korea(NRF) - Korean government (Ministry of Science and ICT(MSIT)) [NRF-2020M3E9A1111636]
- KIST Institutional Program [2E31281]
- Cooperative Research Program for Agriculture Science and Technology Development Rural Development Administration [PJ014790022021]
- Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program [1711134045]
- Korea Evaluation Institute of Industrial Technology (KEIT) [20012362] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Council of Science & Technology (NST), Republic of Korea [KGM2112133] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2021M3A9I5021439] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In this study, a high-performance chemical receptor-conjugated graphene electronic nose (CRGE-nose) was developed for in-situ monitoring of cadaverine (CV) and putrescine (PT) concentration changes in spoiled food.
Monitoring food spoilage is one of the most effective methods for preventing food poisoning caused by biogenic amines or microbes. Therefore, various analytical techniques have been introduced to detect low concentrations of cadaverine (CV) and putrescine (PT), which are representative biogenic polyamines involved in food spoilage (5-8 ppm at the stage of initial decomposition after storage for 5 days at 5 degrees C and 17-186 ppm at the stage of advanced decomposition after storage for 7 days at 5 degrees C). Although previous methods showed selective CV and PT detection even at low concentrations, the use of these methods remains challenging in research areas that require in-situ, real-time, on-site monitoring. In this study, we demonstrated for the first time an in-situ high-performance chemical receptor-conjugated graphene electronic nose (CRGE-nose) whose limits of detection (LODs), 27.04 and 7.29 ppb, for CV and PT are up to 10(2) times more sensitive than those of conventional biogenic amine sensors. Specifically, the novel chemical receptors 2,7-bis(3-morpholinopropyl)benzo[lmn][3,8] phenanthroline-1,3,6,8(2H,7H)-tetraone (NaPhdiMor (NPM)) and 2,7-bis(2-((3-morpholinopropyl)amino)ethyl) benzo[lmn][3,8]phenanthroline-1,3,6,8(2H,7H)-tetraone (NaPhdiEtAmMor (NPEAM)) were designed on the basis of density functional theory (DFT) calculations, and their interaction mechanism was characterized by a DFT 3D simulation. Interestingly, the CRGE-nose was connected on a micro sim chip substrate via wire bonding and then integrated into wireless portable devices, resulting in a cost-effective, high-performance prototype CRGE-nose device capable of on-site detection. The portable CRGE-nose can be used for in-situ monitoring of CV and PT concentration changes as low as 27.04 and 7.29 ppb in real meats such as pork, beef, lamb and chicken.
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