4.8 Article

Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles

期刊

BIORESOURCE TECHNOLOGY
卷 353, 期 -, 页码 -

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ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2022.127140

关键词

Lignocellulose; Hot water pretreatment; Microspectrometry; Fluorescence; FTIR microspectroscopy

资金

  1. Grand-Est
  2. Grand Reims

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This work provides a dynamic perspective on the enzymatic degradation of maize cell walls and sheds new light on the recalcitrance of hot water pretreated maize stem internodes. Depending on their polymer composition and organization, different cell types exhibit varying degrees and rates of enzymatic degradation. Enzymes sequentially act from cell walls rich in accessible cellulose to the most recalcitrant cells, and this phenomenon may be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with cell type.
This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes. Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale. Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation. Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells. This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.

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