Aerobic composting of chicken manure with penicillin G: Community classification and quorum sensing mediating its contribution to humification

Chicken manure containing antibiotics is a hazardous biological waste. The purpose of our study was to investigate how different concentrations of penicillin G alter the bacterial community to affect humification during aerobic composting of chicken manure. The effect of quorum sensing on the bacterial community was also evaluated. Penicillin G mainly affects low fold changes (within 4) for low-abundance (within 200) microbial genera. We found that the bacterial community cooperated to regulate humus and humic acid synthesis. The microbial genera that make up the bacterial community are different, but each bacterial community may have the same ecological function. Quorum sensing affects humic acid synthesis by regulating carbohydrate metabolism and amino acid metabolism in bacterial communities through mechanisms such as the pentose phosphate pathway and the shikimate pathway. This work presents an understanding of the impact of quorum sensing on the collaboration between bacterial communities during composting.

Automated summary

The presence of antibiotics in chicken manure can disrupt the collaboration between bacterial communities and their ability to regulate humification. Penicillin G mainly affects low-abundance microbial genera, but different bacterial communities may have similar ecological functions. Quorum sensing influences humic acid synthesis by regulating carbohydrate and amino acid metabolism in bacterial communities.