期刊
BIOMEDICINE & PHARMACOTHERAPY
卷 150, 期 -, 页码 -出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2022.112948
关键词
Diabetic ulcers; Traditional Chinese medicine; Inflammation; Berberine; IL-17
资金
- National Key RD Plan [2019YFC1708900]
- National Natural Science Foundation of China [81974550]
- Scientific and Technological Innovation project of China Academy of Chinese Medical Science [CI2021A04612]
- Fundamental Research Funds for the Central Public Welfare Research Institute [ZZ13-YQ-046, ZXKT21007]
The study found that inhibiting the IL-17 signaling pathway can promote wound healing in diabetic ulcers, while HB and berberine have important roles in promoting wound healing in type 2 diabetes mellitus.
Diabetic ulcer is a challenging complication of diabetes mellitus but current treatments cannot achieve satisfactory results. In this study, the effect of Huangbai liniment (HB) and berberine on the wound healing in high fat diet/streptozotocin injection induced diabetic rats was investigated by RNA-seq technology. HB topical treatment promoted wound healing in the diabetic patients and diabetic rats, and it affected multiple processes, of which IL-17 signalling pathway was of importance. Inhibiting IL-17a by its inhibitor or antibody remarkably facilitated wound healing and HB significantly repressed the high IL-17 expression and its downstream targets, including Cxcl1, Ccl2, Mmp3, Mmp9, G-CSF, IL1B and IL6, in diabetic wounds, promoted T-AOC, SOD activity and GSH levels; decreased the levels of nitrotyrosine and 8-OHdG; enhanced angiogenesis-related CD31, PDGFBB and ANG1 expression; inhibited cleaved caspase-3 levels and promoted TIMP1 and TGFB1. Moreover, berberine (a major component in HB) repressed the IL-17 signalling pathway, and promoted wound healing in diabetes mellitus. This study highlights the strategy of targeting IL-17a in diabetic wounds, deepens the understanding of wound healing in diabetes mellitus in a dynamic way and reveals the characteristics of HB and berberine in promoting wound healing of type 2 diabetes mellitus.
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