Application of a Mytilus edulis-derived promoting calcium absorption peptide in calcium phosphate cements for bone

The IEELEEELEAER peptide (PIE) identified from the protein hydrolysate of Mytilus edulis is reported to enhance osteoblast growth and differentiation, which also possesses a superior bone formation ability both in vitro and in vivo. Moreover, PIE bound to calcium spontaneously at the stoichiometry of 1:1, and there were amino nitrogen and carboxyl oxygen atoms in 2 glutamic acid residues at the calcium-binding sites in the PIE. The PIE-calcium complex facilitated calcium uptake through the Caco-2 cell monolayers. Incorporation of PIE into calcium phosphate cements enhanced calcium ion uptake and proliferation of osteoblasts and inhibit bacteria. This study suggest that calcium phosphate cements supplemented with PIE can serve as a potentially efficient material for bone graft used during spinal surgery.

Automated summary

The IEELEEELEAER peptide (PIE) derived from Mytilus edulis protein hydrolysate enhances osteoblast growth and differentiation and has a superior bone formation ability. It binds to calcium and facilitates calcium uptake, and when incorporated into calcium phosphate cements, it promotes calcium ion uptake, osteoblast proliferation, and inhibits bacterial growth. This study suggests that PIE-supplemented calcium phosphate cements can be used as an efficient material for bone grafts in spinal surgery.