期刊
BIOLOGICAL PSYCHIATRY
卷 92, 期 12, 页码 984-998出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.biopsych.2022.04.019
关键词
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资金
- National Key R&D Program of China [2022ZD0204702]
- National Natural Science Foundation of China [3183003, 82090032]
- Key Area Research and Development Program of Guangdong Province [2018B030334001, 2018B030340001]
- Science and Technology Program of Guangzhou [202007030013]
This study investigates the role of astrocytic CHRM1 in the regulation of adult hippocampal neurogenesis (AHN) and memory. The results show that genetic ablation of CHRM1 in astrocytes leads to defects in neural stem cell survival, neuronal differentiation, and maturation and integration of newborn neurons in the dentate gyrus. Astrocytic CHRM1-mediated modulation of AHN is mediated by BDNF signaling. Furthermore, CHRM1 ablation in astrocytes impairs contextual fear memory. These impairments in both AHN and memory are rescued by overexpression of astrocytic CHRM1 in the dentate gyrus.
BACKGROUND: In the neurogenic niches of the adult hippocampus, new functional neurons are continuously generated throughout life, and generation of these neurons has been implicated in learning and memory. Astrocytes, as components of the neurogenic niches, are critical in the regulation of adult hippocampal neurogenesis (AHN). However, little is known about how astrocytes receive and respond to extrinsic cues to regulate AHN. METHODS: By using a transgenic strategy to conditionally delete astrocytic CRHM1 in mice and AAV (adenoassociated virus)-mediated overexpression of astrocytic CHRM1 specifically in the hippocampal dentate gyrus, we systematically investigated the role of astrocytic CHRM1 in the regulation of AHN and the underlying mechanisms using the combined approaches of immunohistochemistry, retrovirus labeling, electrophysiology, primary astrocyte cultures, immunoblotting, and behavioral assays. RESULTS: We report that genetic ablation of CHRM1 in astrocytes led to defects in neural stem cell survival, neuronal differentiation, and maturation and integration of newborn neurons in the dentate gyrus. Astrocytic CHRM1-mediated modulation of AHN was mediated by BDNF (brain-derived neurotrophic factor) signaling. Furthermore, CHRM1 ablation in astrocytes impaired contextual fear memory. These impairments in both AHN and memory were rescued by overexpression of astrocytic CHRM1 in the dentate gyrus. CONCLUSIONS: Our findings reveal a critical role for astrocytes in mediating cholinergic regulation of AHN and memory through CHRM1.
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