期刊
BIOCONJUGATE CHEMISTRY
卷 33, 期 5, 页码 969-981出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.2c00167
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类别
资金
- NCI [T32 CA196561]
Lipid-based formulations, combined with magnetic nanoparticles, have shown great potential in biomedical applications such as drug delivery and therapy. In this study, we developed a simplified method to synthesize drug-loaded magnetic multimicelle aggregates and achieved molecular targeting through antibody conjugation.
Lipid-based formulations provide a nanotechnology platform that is widely used in a variety of biomedical applications because it has several advantageous properties including biocompatibility, reduced toxicity, relative ease of surface modifications, and the possibility for efficient loading of drugs, biologics, and nanoparticles. A combination of lipid-based formulations with magnetic nanoparticles such as iron oxide was shown to be highly advantageous in a growing number of applications including magnet-mediated drug delivery and image-guided therapy. Currently, lipid-based formulations are prepared by multistep protocols. Simplification of the current multistep procedures can lead to a number of important technological advantages including significantly decreased processing time, higher reaction yield, better product reproducibility, and improved quality. Here, we introduce a one-pot, single-step synthesis of drug-loaded magnetic multimicelle aggregates (MaMAs), which is based on controlled flow infusion of an iron oxide nanoparticle/lipid mixture into an aqueous drug solution under ultrasonication. Furthermore, we prepared molecular-targeted MaMAs by directional antibody conjugation through an Fc moiety using Cu-free click chemistry. Fluorescence imaging and quantification confirmed that antibody-conjugated MaMAs showed high cell-specific targeting that was enhanced by magnetic delivery.
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