期刊
BIOCHIP JOURNAL
卷 16, 期 2, 页码 197-206出版社
KOREAN BIOCHIP SOCIETY-KBCS
DOI: 10.1007/s13206-022-00058-z
关键词
Chronic diseases; LDL detection; Non-faradaic; Real-time monitoring
资金
- Taif University, Taif, Saudi Arabia [TURSP-2020/264]
In this study, a non-faradaic impedimetric biosensor was developed for the direct detection of low-density lipoprotein (LDL). The biosensor showed high sensitivity and low limit of detection, and demonstrated good selectivity towards LDL. It was also used for continuous monitoring of LDL, which is significant for direct real-time monitoring and early medical interventions in chronic diseases.
In this work, non-faradaic impedimetric biosensor was developed for the direct detection of low-density lipoprotein (LDL-cholesterol). Unlike other electrochemical techniques, non-faradaic electrochemical impedance spectroscopy (EIS) does not require the use of redox probes which denatures targeted biomarkers. Furthermore, no reference electrode is required for detection which is commercially viable. The biosensor reported here consists of LDL-antibodies attached to interdigitated gold electrodes via standard chemical functionalization. The biosensor demonstrated a sensitivity and limit of detection (LoD) of 70 nF/log(ng/mL) and 120 pg/mL, respectively, where the LoD is well below the recommended LDL level in blood. The biosensor also demonstrated good selectivity toward LDL. Thereafter we utilized, for the first time, the non-faradaic impedimetric biosensor for the continuous monitoring of LDL in phosphate buffer saline (PBS). The biosensor showed rapid responses to LDL injections. Such an approach is essential for the development of low-cost and scalable wearable biosensors for the direct real-time monitoring of chronic diseases and hence allows early medical interventions in cases where sudden increases in LDL or other biomarkers are deadly.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据