A new method for primary culture of microglia in rats with spinal cord injury

At present, the primary culture method of microglia is complicated, and the culture of spinal cord microglia is rare, so we will explore to establish a new and efficient primary culture method of microglia in rats with spinal cord injury (SCI). The SCI model of SD rats was established by modified A11en0s method, and the model of SCI was performed on 1 d, 3 d, 7 d and 14 d respectively. Then the injured spinal cord was removed, mechanically separated and filtered. The morphology of microglia was observed the next day and its purity was identified by CD11b and Iba1 immunofluorescence labeling. According to the above results, the morphological changes of microglia after 3 d of SCI were observed at 1 d, 2 d and 4 d. The results showed that the purity of microglia was 98%. The number of microglia after 3 d of SCI was the most. After SCI, the migration ability of microglia was enhanced, the number of microglia in the injured area increased, and the number was the highest at 3 d, then gradually decreased. In addition, the microglia after SCI would gradually change from active state to resting state with the passage of time. Therefore, we can use a simple and efficient mechanical separation method to extract primary microglia, which provides the basis for the study of microglia. (c) 2022 Elsevier Inc. All rights reserved.

Automated summary

This study aims to establish a new and efficient primary culture method for microglia in rats with spinal cord injury (SCI). The SCI model was created in SD rats using a modified A11en0s method, and microglia were observed and separated at different time points. The results showed that the number and purity of microglia were the highest after 3 days of SCI, and microglia exhibited a transition from active to resting state.