期刊
BEHAVIOURAL BRAIN RESEARCH
卷 426, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbr.2022.113847
关键词
Lactococcus lactis subsp; cremoris; Depression; LPS; Forced swim test; Anti-inflammatory activity
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brasil (CAPES) [001]
- National Council for Scientific and Technological Development, Brasil (CNPQ) [304096/2020-2]
- Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul, Brasil (FAPERGS)
This study evaluated the effectiveness of a probiotic intervention using Lactococcus lactis subsp. cremoris LL95 in ameliorating mood disorders in a lipopolysaccharide (LPS)-induced depression-like mouse model. The results showed that LL95 intervention improved LPS-induced depression-like behaviors in mice and modulated oxidative and inflammatory markers in the hippocampus, as well as altered the LAB content of the gut microbiota.
Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression. This study evaluated the effectiveness of a probiotic intervention using Lactococcus lactis subsp. cremoris LL95 in ameliorating mood disorders in a lipopolysaccharide (LPS)-induced depression-like mouse model. C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 x 10(9) CFU/mouse). The animals were then subjected to a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests. In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-alpha, and interleukin-1 beta in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content. LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test. LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-alpha, and of interleukin-1 beta to a lesser extent. Furthermore, LL95 intervention increased the fecal LAB content in these animals, suggesting changes in the gut microbiota. These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
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