期刊
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
卷 42, 期 3, 页码 243-252出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/ATVBAHA.121.315849
关键词
atherosclerosis; cardiovascular disease; cholesterol; macrophage; proteomics; transcriptomics
资金
- NIH-NHLBI [R01 HL153712-01]
- AHA [20SFRN35210252, NFL-2020-218415]
The development of innovative single-cell technologies has allowed for in-depth analysis of the transcriptomic and proteomic profiling of individual blood and tissue cells. Recent single-cell studies have revealed the cellular heterogeneity of atherosclerotic plaque tissue and provided a better understanding of the immune functional states in the context of atherosclerosis.
The development of innovative single-cell technologies has allowed the high-dimensional transcriptomic and proteomic profiling of individual blood and tissue cells. Recent single-cell studies revealed a new cellular heterogeneity of atherosclerotic plaque tissue and allowed a better understanding of distinct immune functional states in the context of atherosclerosis. In this brief review, we describe how single-cell technologies have shed a new light on the cellular composition of atherosclerotic plaques, and their response to diet perturbations or genetic manipulation in mouse models of atherosclerosis. We discuss how single-cell RNA sequencing, cellular indexing of transcriptomes and epitopes by sequencing, transposase-accessible chromatin with high-throughput sequencing, and cytometry by time-of-flight platforms have empowered the identification of discrete immune, endothelial, and smooth muscle cell alterations in atherosclerosis progression and regression. Finally, we review how single-cell approaches have allowed mapping the cellular and molecular composition of human atherosclerotic plaques and the discovery of new immune alterations in plaques from patients with stroke.
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