Exosomes from human umbilical cord mesenchymal stem cells and human dental pulp stem cells ameliorate lipopolysaccharide-induced inflammation in human dental pulp stem cells

Objective: The aim of this study was to evaluate the effects of exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSCs-Exo) and human dental pulp stem cells (hDPSCs-Exo) on lipopolysaccharideinduced inflammation in human dental pulp stem cells. Methods: Exosomes were extracted from the supernatant of human umbilical cord mesenchymal stem cells and human dental pulp stem cells. Lipopolysaccharide was used to establish the inflammation model in human dental pulp stem cells. Then human dental pulp stem cells were treated with hUCMSCs-Exo and hDPSCs-Exo, respectively. We examined the proliferation, apoptosis, and secretion of inflammatory cytokines of human dental pulp stem cells in vitro. Results: Exosomes had the capacity to reverse the lipopolysaccharide-induced inhibitory effect on human dental pulp stem cells proliferation and suppress lipopolysaccharide-induced apoptosis. Additionally, exosomes reduced the pro-inflammatory cytokines expression and increased anti-inflammatory cytokine expression. However, compared with hDPSCs-Exo, hUCMSCs-Exo had better ability to reverse inhibition of proliferation, suppress apoptosis and regulate release of inflammatory cytokines in human dental pulp stem cells. Conclusions: Our data suggest that exosomes could alleviate inflammation in human dental pulp stem cells. hUCMSCs-Exo do show even stronger effects than hDPSCs-Exo.

Automated summary

This study evaluated the effects of exosomes derived from human umbilical cord mesenchymal stem cells and human dental pulp stem cells on lipopolysaccharide-induced inflammation in human dental pulp stem cells. The results showed that exosomes could reverse the inhibitory effect of lipopolysaccharide, suppress apoptosis, and reduce the expression of pro-inflammatory cytokines.