期刊
ARCHIVES OF MICROBIOLOGY
卷 204, 期 5, 页码 -出版社
SPRINGER
DOI: 10.1007/s00203-022-02850-9
关键词
Biodegradation; Pyrene; Polycyclic aromatic hydrocarbons; Contaminated soil; Petroleum
类别
资金
- King Abdul-Aziz City for Science and Technology (KACST) [1-17-01-010-0012]
In this study, a strain of Klebsiella oxytoca with efficient pyrene degradation ability was isolated from petroleum-contaminated soil. Through various experiments and analysis, the mechanism and pathway of pyrene degradation by K. oxytoca were elucidated, demonstrating its potential for soil aromatic hydrocarbon removal.
Polycyclic aromatic hydrocarbons (PAHs) are the hazardous xenobiotic agents of oil production. One of the methods to eliminate hazardous compounds is bioremediation, which is the most efficient and cost-effective method to eliminate the harmful byproducts of crude petroleum processing. In this study, five pure bacterial isolates were isolated from petroleum-contaminated soil, four of which showed a robust growth on the PAH pyrene, as a sole carbon source. Various methods viz mass spectroscopy, biochemical assays, and 16S RNA sequencing employed to identify the isolates ascertained the consistent identification of Klebsiella oxytoca by all three methods. Scanning electron microscopy and Gram staining further demonstrated the characterization of the K. oxytoca. High-performance liquid chromatography of the culture supernatant of K. oxytoca grown in pyrene containing media showed that the cells started utilizing pyrene from the 6th day onwards and by the 12th day of growth, 70% of the pyrene was completely degraded. A genome search for the genes predicted to be involved in pyrene degradation using Kyoto Encyclopedia of Genes and Genomes (KEGG) confirmed their presence in the genome of K. oxytoca. These results suggest that K. oxytoca would be a suitable candidate for removing soil aromatic hydrocarbons.
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