Intraperitoneal injection of 17 beta-estradiol increases ovarian smad2/3 expression in Yangtze sturgeon Acipenser dabryanus

Smad (mothers against decapentaplegic homologue) proteins are the downstream signalling factors involved in TGF beta (transforming growth factor beta) pathways. The present study was aimed to reveal the estradiol feedback regulation of Smad signalling in one of the endangered sturgeon species Acipenser dabyanus. Two receptor-activated (R-) smad paralogues, smad2 and smad3 were identified in Yangtze sturgeon, whose full-length cDNA sequences were 1,824 and 1,876 base pairs, encoding 467 and 426 amino acids respectively. Multiple sequence alignment suggested that both Smad2 and Smad3 were highly conserved among vertebrates. Phylogenetic analysis indicated that Smad2 was more conserved than Smad3 in Yangtze sturgeon. Tissue distribution analysis revealed that both smad2 and smad3 transcribed ubiquitously in heart, liver, spleen, kidney, intestine, testis, ovary, midbrain and pituitary. Transcription levels of smad2 and smad3 in the ovary were increased significantly after 17 beta-estradiol (E2) intraperitoneal injection (p < 0.05). Besides, transcriptions of aromatase (cyp19a1a), and oestrogen receptor beta (er beta) were decreased due to E2 injection (p < 0.05). These results indicate that E2 exerts positive feedback effect on R-smad signalling, potentially through the downregulation of aromatase and ER beta, in the ovary of sturgeons.

Automated summary

This study reveals the positive feedback regulation of Smad signaling by estradiol in the ovaries of endangered sturgeon species Acipenser dabyanus, and also identifies the widespread expression of R-smad signaling factors in multiple tissues.