期刊
AQUACULTURE
卷 551, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2022.737943
关键词
Cyprinid herpesvirus 3 (CyHV-3); Cyclophilin A; CyHV-3 VP32; Antiviral agents
资金
- National Key Research and Develop-ment Program of China [2018YFD0900501]
- Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) [311021006]
- Key Research and Development Program of Zhuhai city
- Guangdong Provincial Special Fund [2019KJ141]
This study revealed the involvement of CypA protein in CyHV-3 replication, as it interacts with the viral envelope protein VP32. Inhibition of CypA reduces viral proliferation, while overexpression enhances it.
Cyprinid herpesvirus 3 (CyHV-3), a large double-stranded nucleocytoplasmic DNA virus, is the major viral pathogen for Cyprinus carpio distributed worldwide. Cyclophilin A (CypA) is a well-characterized ubiquitous cellular immunosuppressor possessing peptidyl-prolyl cis-trans isomerase activity in many species and plays an important role in regulating the replication of several well-known viruses. In our previous study, C. carpio CypA was detected as a high confident cellular protein involvement in purified extracellular virion of CyHV-3, however, the exact role of C. carpio CypA in CyHV-3 remains unclear. Here we identified that C. carpio CypA was incorporated into mature CyHV-3 virion and located in envelope fraction. Co-Immunoprecipitation and subcellular co localization showed that C. carpio CypA interacted with CyHV-3 VP32, an essential viral envelope protein for CyHV-3 replication. Drug inhibition assay showed that knocking down of C. carpio CypA significantly reduced CyHV-3 proliferation in KCF-1 cells, whereas overexpression of C. carpio CypA enhanced CyHV-3 replication. Subcellular locations of C. carpio CypA showed that both exogenous and endogenous CypA were found to translocate from cytoplasm to whole cell upon CyHV-3 infection. Collectively, our results confirmed that C. carpio CypA was incorporated into CyHV-3 virion via interaction with CyHV-3 VP32 and played an essential role in CyHV-3 replication. C. carpio CypA is suggested as a potential target cellular protein for developing antiviral agents against CyHV-3 infection.
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