Modification of the Chemical Structure, Morphology, and Cytocompatibility of Chitosan Films via Low-Frequency Plasma Treatment

The effect of low-frequency plasma treatment of chitosan films on the surface chemical structure, morphology, and properties, including the ability to support adhesion and the proliferation of human keratinocytes, was evaluated. It was shown that plasma treatment led to the formation of polar oxygen-containing groups on the surface, a decrease in surface roughness, and an increase in surface hydrophilicity. The chitosan film samples were cytocompatible, and they maintained keratinocyte viability when used as extracts or as supports for cell cultivation. Plasma treatment of chitosan films increased their adhesion properties with respect to cells and activated keratinocyte proliferation, which is important for the application of chitosan-containing materials as a wound dressing.

Automated summary

This study evaluated the effect of low-frequency plasma treatment on the surface properties of chitosan films and its impact on the adhesion and proliferation of human keratinocytes. The results showed that plasma treatment resulted in the formation of polar oxygen-containing groups on the surface, reduced surface roughness, and increased surface hydrophilicity. The chitosan films exhibited cytocompatibility and supported keratinocyte viability in extract or cell cultivation applications. Plasma treatment enhanced the adhesion properties of chitosan films to cells and stimulated keratinocyte proliferation, which is crucial for the use of chitosan-containing materials as wound dressings.