Hydrophilized Ultrafiltration Membranes Synthesized from Acrylic Acid Grafted Polyethersulfone for Downstream Processing of Therapeutic Insulin and Cobalamin

The present study focuses on synthesis of novel high-performance acrylic acid (AA) grafted polyethersulfone (PES) ultrafiltration (UF) membranes for purification of small therapeutic biomolecules such as urea, insulin, and cobalamin. The membranes were indigenously synthesized by adding polyethylene glycol (PEG) of 6 kDa M.Wt. as a pore former and subsequent grafting of AA using 2 to 6 wt.% concentrations under UV-induced photo grafting. Scanning electron microscopy reveals that the PEG additive profoundly influences the pore density on the membrane surface. FTIR spectra confirm the graft polymerization of AA with the PES substrate. Separation performance of the grafted membranes was evaluated to establish the trade-off between the degree of grafting and MWCO. From the experimental results, the pure water flux (PWF) of 6% grafted PES membrane was enhanced from 8.5 (PES [0] [6]) to 18.20 l m(-2) h(-1) (PES [6 +] [6]) in the presence of PEG pore former, respectively. The grafting concentration window of 2-6% resulted in selective membranes to altogether remove uremic toxins into the permeate with retention of high molecular size proteins. Hence, 5 and 6 wt.% AA grafted membranes exhibited > 90% rejection for insulin and cobalamin biomolecules along with 24.5 and 23.8 l m(-2) h(-1) bar(-1) permeability towards urea, respectively. The process results correlate well with the MWCO values of membranes ranging from 1 to 10 kDa. This work provides the efficacy of these grafted membranes for potential application in the downstream processing of therapeutic biomolecules such as insulin and cobalamin.

Automated summary

The present study focuses on synthesizing novel high-performance acrylic acid grafted polyethersulfone (PES) ultrafiltration (UF) membranes for purification of small therapeutic biomolecules. The membranes were synthesized by adding polyethylene glycol (PEG) as a pore former and grafting acrylic acid (AA) onto PES under UV-induced photo grafting. Scanning electron microscopy revealed that PEG additive greatly influenced the pore density on the membrane surface. The membranes showed improved pure water flux and selective removal of uremic toxins, while maintaining high rejection rates for insulin and cobalamin biomolecules.