In Vitro Bedaquiline and Clofazimine Susceptibility Testing in Mycobacterium abscessus

Bedaquiline and clofazimine are increasingly used to treat infections with Mycobacterium abscessus. We determined distributions of MICs by broth microdilution for bedaquiline and clofazimine for 61 M. abscessus clinical isolates using different media and incubation times. We show that incubation time and growth media critically influence the MIC. Our data will aid in defining future clinical breakpoints for in vitro susceptibility testing for bedaquiline and clofazimine in M. abscessus.

Automated summary

In this study, we investigated the distribution of minimum inhibitory concentrations (MICs) of bedaquiline and clofazimine for 61 clinical isolates of M. abscessus using different media and incubation times. Our findings demonstrate that incubation time and growth media play a critical role in determining the MIC. This information will be valuable for establishing clinical breakpoints for in vitro susceptibility testing of bedaquiline and clofazimine in M. abscessus.