期刊
ANTICANCER RESEARCH
卷 42, 期 5, 页码 2487-2493出版社
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.15727
关键词
SREBP1; galectin-3; thyroid cancer
类别
资金
- Ministry of Science and Technology of Taiwan [MOST-110-2314-B-195-018-MY3]
- MacKay Memorial Hospital [MMH-11116, MMH-E-111-08]
The study found that the expression of SREBP1 in thyroid cancer cells is affected by various specific inhibitors, among which, Lenvatinib and a MEK inhibitor can suppress SREBP1 expression in BRAF mutant cells, while a galectin-3 inhibitor can reduce SREBP1 expression in both cells in a time- and dose-dependent manner.
Background/Aim: Expression of sterol regulatory element-binding protein 1 (SREBP1) is upregulated in thyroid cancer and associated with shorter disease-specific survival. The molecular regulatory mechanisms governing SREBP1 over-expression in thyroid cancer are still unclear. Materials and Methods: Thyroid cancer cell lines BHT-101 (with the BRAF V600E mutation) and FTC-131 (wild-type for BRAF) were treated with specific inhibitors. The expression of SREBP1 was determined at the mRNA level using quantitative real-time PCR and at the protein level using immunoblotting. Results: Lenvatinib and a MEK inhibitor, selumetinib, suppressed SREBP1 expression in BHT-101 but not FTC-133 cells. Olitigaltin, a galectin-3 inhibitor, decreased SREBP1 expression in a time-and dose-dependent manner in both cells. MK2206, an allosteric AKT inhibitor, did not change SREBP1 expression in either cell line. Conclusion: The galectin-3 inhibitor attenuates SREBP1 expression in thyroid cancer cells, likely independent of AKT phosphorylation. Lenvatinib and selumetinib decreases SREBP1 expression in the BRAF-mutant cell line BHT-101.
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