期刊
ANIMAL REPRODUCTION SCIENCE
卷 241, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.anireprosci.2022.106991
关键词
Sperm storage; Carp; Reactive oxygen species; Sperm motility; Short-term
资金
- Ministry of Education, Youth and Sports of the Czech Republic [LM2018099, CZ.02.1.01/0.0/0.0/16_025/0007370]
- CAS President's International Fellowship [2017PB0060]
- National Natural Science Foundation of China [21676286]
- Primary Research Development Plan of Shandong Province [2016GSF121006]
This study investigated the effects of refrigerated storage on sperm quality and oxidative stress in common carp. After 72 hours of storage, sperm motility significantly decreased, and after 144 hours, sperm motility was less than 40%. The levels of lipid peroxidation and protein carbonyl derivatives, indicators of oxidative stress, increased over time.
Short-term storage of semen is a simple and inexpensive procedure to deal with logistics of large-scale hatchery operations but can lead to oxidative stress and a significant decrease in sperm motility and velocity. To better understand the mechanisms responsible for the association of poor sperm quality with oxidative stress, in the present study we investigated the effect of refrigerated storage for 0, 24, 48, 72, and 144 h on sperm motility and curvilinear velocity and oxidant/antioxidant balance of common carp Cyprinus carpio. Percentage of motile sperm was significantly (p < 0.05) reduced stored for 72 h storage compared to that of fresh sperm. Sperm stored 144 h showed < 40% motility with an average velocity of 91.12 +/- 10.4 mu m s(-1). A time dependent increase in the level of the oxidative stress indices lipid peroxidation and carbonyl derivatives of proteins was observed. Increase (p > 0.05) in total superoxide dismutase was detected after 48 h, and glutathione reductase and glutathione peroxidase activity demonstrated a significant increase after 72 h. These results provide an additional tool for the development and improvement of short-term sperm preservation procedures commonly applied in aquaculture.
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