期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 23, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202201929
关键词
DNA Nanotechnology; Dissipative Self-Assembly; Strand Displacement Reaction; Temporal Control
资金
- European Research Council, ERC [819160, 724863]
- Associazione Italiana per la Ricerca sul Cancro, AIRC [21965]
- Italian Ministry of Health [GR-2013-02356714]
- Italian Ministry of University and Research [2017YER72K]
- Deutsche Forschungsgemeinschaft (DFG) [SE 1646/9-1, 2141]
- European Union [896962]
- Italian Ministry of Education and Research [2017E44A9P]
- Projekt DEAL
- Marie Curie Actions (MSCA) [896962] Funding Source: Marie Curie Actions (MSCA)
- European Research Council (ERC) [724863] Funding Source: European Research Council (ERC)
This study presents a general approach to achieve dissipative control over toehold-mediated strand-displacement, a widely used reaction in DNA nanotechnology. By re-engineering the classic reaction, the high-energy invader strand is converted into a low-energy waste product, allowing the system to spontaneously return to its original state over time. This method enables unique temporal activation of DNA systems and is reversible and highly controllable.
Here we show a general approach to achieve dissipative control over toehold-mediated strand-displacement, the most widely employed reaction in the field of DNA nanotechnology. The approach relies on rationally re-engineering the classic strand displacement reaction such that the high-energy invader strand (fuel) is converted into a low-energy waste product through an energy-dissipating reaction allowing the spontaneous return to the original state over time. We show that such dissipative control over the toehold-mediated strand displacement process is reversible (up to 10 cycles), highly controllable and enables unique temporal activation of DNA systems. We show here two possible applications of this strategy: the transient labelling of DNA structures and the additional temporal control of cascade reactions.
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