期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 20, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202116482
关键词
MicroRNA; MspA; Nanopores; Nucleic Acid Barcode; Proteins
资金
- National Natural Science Foundation of China [31972917, 91753108, 21675083]
- Fundamental Research Funds for the Central Universities [020514380257, 020514380261]
- Programs for high-level entrepreneurial and innovative talents introduction of Jiangsu Province
- Natural Science Foundation of Jiangsu Province [BK20200009]
- Excellent Research Program of Nanjing University [ZYJH004]
- Shanghai Municipal Science and Technology Major Project, State Key Laboratory of Analytical Chemistry for Life Science [5431ZZXM1902]
- Technology innovation fund program of Nanjing University, China Postdoctoral Science Foundation [2021M691508]
This study demonstrates a barcode system based on self-assembly of synthetic nucleic acids and direct nanopore decoding. Each barcode is composed of multiple information nodes, which can be scanned and decoded sequentially. This system can be applied to detect various antibody proteins or cancer-related microRNAs.
A large collection of unique molecular barcodes is useful in the simultaneous sensing or screening of molecular analytes. Though the sequence of DNA has been widely applied to encode for molecular barcodes, decoding of these barcodes is normally assisted by sequencing. We here demonstrate a barcode system based solely on self-assembly of synthetic nucleic acids and direct nanopore decoding. Each molecular barcode is composed of n distinct information nodes in a non-binary manner and can be sequentially scanned and decoded by a Mycobacterium smegmalis porin A (MspA) nanopore. Nanopore events containing step-shaped features were consistently reported. 14 unique information nodes were developed which in principle could encode for 14(n) unique molecular barcodes in a barcode containing n information nodes. These barcode probes were adapted to detect different antibody proteins or cancer-related microRNAs, suggesting their immediate application in a wide variety of sensing applications.
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