期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 24, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202117436
关键词
Counterion; Multiplexed Imaging; NIR-II; Polymethine Cyanine Fluorophores
资金
- National Natural Science Foundation of China (NSFC) [22088101, 21725502, 51961145403, 21904023, 21908030, 22004018]
- Research Program of Science and Technology Commission of Shanghai Municipality [20JC1411700, 19490713100, 20490710600, 20S31903700]
This study developed a series of heptamethine cyanines with varied absorption/emission maxima in the NIR-II spectral window. The utilization of bulky counterions significantly improved absorptivity, brightness, and spectral profiles in water, addressing the quenching problem of NIR-II cyanines. The developed cyanines showed promising potential for non-invasive bioimaging in various applications.
Photon excitation and emission at the NIR-II spectral window enable high-contrast deep-tissue bioimaging. However, multiplexed imaging with NIR-II excitation and emission has been hampered by the limited chemical strategies to develop bright fluorophores with tunable absorption in this spectral regime. Herein, we developed a series of heptamethine cyanines (HCs) with varied absorption/emission maxima spanning from 1100 to 1600 nm through a physical organic approach. A bulky counterion paired to HCs was found to elicit substantial improvements in absorptivity (7-fold), brightness (14-fold), and spectral profiles in water, addressing a notorious quenching problem of NIR-II cyanines due to aggregation and polarization. We demonstrated the utilities of HC1222 and HC1342 for high-contrast dual-color imaging of circulatory system, lymphatic structures, tumor, and organ function in living mice under 1120 nm and 1319 nm excitation, showing HCs as a promising platform for non-invasive bioimaging.
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