期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 27, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202203184
关键词
Activatable Probe; Cancer Immunotherapy; Fluorescence Imaging; Photoacoustic Imaging; Tumor-Associated Neutrophils
资金
- National Key Research and Development Program of China [2021YFA1101500]
- Singapore Ministry of Education [2019-T1-002-045, RG125/19, RT05/20, MOE2018-T2-2-042, MOE-T2EP30220-0010]
This study presents an activatable semiconducting polymer nanoprobe for imaging tumor-associated neutrophils using near-infrared fluorescence and photoacoustic imaging. The nanoprobe reacts with neutrophil elastase to enhance imaging signals and correlate with the intratumoral population of TANs.
Imaging to evaluate tumor-associated neutrophils (TANs) is imperative for cancer immunotherapy but remains challenging. We herein report an activatable semiconducting polymer nanoprobe (SPCy) for near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging of neutrophil elastase (NE), a biomarker of TANs. SPCy comprises a semiconducting polymer conjugated with a hemicyanine (hemi-Cy) dye caged by a NE-cleavable peptide as the side chain. After systemic administration, SPCy passively targets the tumor and reacts with NE to uncage the hemi-Cy, leading to enhanced NIRF and PA signals of the hemi-Cy but unchanged signals of the SP. Such NE-activated ratiometric NIRF and enhanced PA signals of SPCy correlate with the intratumoral population of TANs. Thus, this study not only presents the first TAN-specific PA probe, but also provides a general molecular design strategy for PA imaging of other immune-related biomarkers to facilitate screening of cancer immunotherapeutics.
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