期刊
ANALYTICAL CHEMISTRY
卷 94, 期 17, 页码 6457-6462出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c00403
关键词
-
资金
- National Natural Sciences Foundation of China [21625504, 21827807, 21790390/21790392]
- Chinese Academy of Sciences
An automatic device called SoniCoat was developed in this study for matrix coating using an ultrasonic nozzle driven by a piezoelectric ceramic. Compared with other commercial and homemade devices, SoniCoat achieved good nebulization effect, generated more homogeneous matrix crystals, and reduced molecular diffusion. Additionally, the newly developed device can be used for in situ derivatization.
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has emerged as a powerful tool for studying the spatial distribution of various types of molecules. Matrix deposition is a critical step for obtaining high-quality imaging data. An automatic device named SoniCoat was fabricated based on an ultrasonic nozzle driven by a pizeoelectric ceramic for matrix coating in the present study. Compared with the minihumidifier developed previously by our group for matrix deposition, SoniCoat realized automation, and the ultrasonic nozzle of this device is easy to clean and less likely to get clogged, indicating a longer life. Compared with commercial and homemade instruments such as the TM-Sprayer and electrospray matrix coating device, our newly developed device achieved a good nebulization effect without the need for high-pressure gas flow and high voltage. Matrix deposition by SoniCoat generated more homogeneous matrix crystals with diameters of about 10 mu m and reduced the occurrence of molecular diffusion to a greater extent, compared with the results by TM-Sprayer. Repeatable high-spatial-resolution MS imaging data were obtained with the help of SoniCoat. Furthermore, the newly developed device can also be successfully applied for in situ derivatization.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据