4.8 Article

Exploring Integrin-Mediated Force Transmission during Confined Cell Migration by DNA-Based Tension Probes

期刊

ANALYTICAL CHEMISTRY
卷 94, 期 11, 页码 4570-4575

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c04962

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资金

  1. National Natural Science Foundation of China [21775115, 32071305, 32150016]
  2. Wuhan University
  3. Fundamental Research Funds for the Central Universities [2042018kf02, 2042021kf0030, 2042018kf1006]
  4. Innovation Funds for Postdocs in Hubei Province

向作者/读者索取更多资源

Mechanical forces have profound effects on cell morphology and migration in a two-dimensional environment. However, the mechanism by which cells migrate in a confined three-dimensional environment is unclear. This study presents a method of fabricating microfluidic chips with DNA-based tension probes to measure force exerted during confined cell migration. The results show that cells exert less force and have increasingly transient interactions in confined spaces.
Mechanical forces have profound effects on the morphology and migration of cells in a two-dimensional environment. However, cells in vivo mostly migrate in three-dimensional space while physically constrained, and the mechanism by which cellular dynamic forces drive migration in this confined environment is unclear. Here, we present a method of fabricating microfluidic chips with integrated DNA-based tension probes to measure spatiotemporal variations in integrin-mediated force exerted during confined cell migration. Using this developed device, we measured the spatial locations, magnitudes, and temporal characteristics of integrin-ligand tension signals in motile cells in different microchannels and found that cells exerted less force and underwent increasingly transitory integrin-ligand interactions when migrating in confined spaces. This study demonstrates that the described method provides insights into understanding the migratory machinery of cells in geometrically confined environment that better mimics physiological conditions.

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