期刊
ANALYTICAL CHEMISTRY
卷 94, 期 10, 页码 4141-4145出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c05614
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Mass spectrometry enables automated analysis of complex samples without labeling. Liquid atmospheric pressure matrix-assisted laser desorption/ionization (LAP-MALDI) allows for rapid sample preparation and high-speed mass spectrometry analysis. This technology achieves a significant increase in analysis speed, has high ion signal stability, and can analyze multiply charged peptide ions at extreme speeds.
Mass spectrometry (MS) allows for automated analysis of complex samples at high resolution without the need for labeling/derivatization. Liquid atmospheric pressure matrix-assisted laser desorption/ionization (LAP-MALDI) enables rapid sample preparation and MS analysis using microtiter-plate formats and high-performing mass spectrometers. We present a step change in high-speed, large-scale MS sample analysis of peptides at 20 samples/s and an enzymatic assay at 40 samples/s, i.e., an order of magnitude faster than current MS platforms. LAP-MALDI requires only low amounts of sample volume (<2 mu L), of which only a fraction (<1%) is typically consumed, and allows for multiplexing and high-speed MS/MS analysis, demonstrated at similar to 10 samples/s. Its high ion signal stability and similarity to electrospray ionization enables CVs below 10% and the analysis of multiply charged peptide ions at these extreme speeds. LAP-MALDI MS fulfills the speed requirements for large-scale population diagnostics and compound screening with the potential of analyzing >1 million samples per day.
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