Long-Term Preservation of SARS-CoV-2 RNA in Silk for Downstream RT-PCR Tests

Article Infectious Diseases

SARS-CoV-2 RNA stability in dry swabs for longer storage and transport at different temperatures

Alonzo Alfaro-Nunez et al.

Summary: This study experimentally evaluated the ability of retaining SARS-CoV-2 in spiked saliva-moist swabs and spiked swabs without transport medium at different environmental settings and incubation time periods. The results showed stable retention of SARS-CoV-2 RNA for up to 9 days at 20 degrees C, and up to 26 days for saliva-moist swabs at -20 degrees C and +4 degrees C, presenting an economical alternative for countries with limited access to rapid refrigerated transport and storage. The study also highlighted the practical and economic advantages of using swabs without transport medium.

TRANSBOUNDARY AND EMERGING DISEASES (2022)

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Article Chemistry, Analytical

A SARS-CoV-2 Reference Standard Quantified by Multiple Digital PCR Platforms for Quality Assessment of Molecular Tests

Haiwei Zhou et al.

Summary: This study utilized digital PCR to estimate the nucleic acid detectable units of an inactivated whole-virus SARS-CoV-2 reference standard candidate, with the quantification results median treated as the consensus true value of virus particles in the standard. The reference standard was then used to challenge the limit of detection of six officially approved diagnostic assays. The study demonstrates that inactivated whole virus quantified by dPCR can serve as a standardized reference for diagnostic assay development, quality control, and regulatory surveillance.

ANALYTICAL CHEMISTRY (2021)

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Article Pathology

Developing multiplex ddPCR assays for SARS-CoV-2 detection based on probe mix and amplitude based multiplexing

Raphael Nyaruaba et al.

Summary: This study demonstrates the development of different ddPCR SAR-CoV-2 assays, including multiplex assays. Results show that the quadruplex assay has similar detection limits and accuracy to lower multiplex assays, and the triplex probe mix assay has better sensitivity than the RT-qPCR assay. Additionally, the ddPCR multiplex assay indicates remdesivir's ability to inhibit SARS-CoV-2 growth in vitro.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2021)

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Article Multidisciplinary Sciences

Chimeric synthetic reference standards enable cross-validation of positive and negative controls in SARS-CoV-2 molecular tests

Bindu Swapna Madala et al.

Summary: DNA synthesis in vitro allows rapid production of reference standards for use as controls in diagnostic tests, improving accuracy and quality. Incorporating both positive and negative controls into diagnostic testing helps evaluate test specificity, while cross-validating these controls in the same reaction distinguishes control and test failures.

SCIENTIFIC REPORTS (2021)

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Article Materials Science, Biomaterials