期刊
ANALYTICAL CHEMISTRY
卷 94, 期 9, 页码 4078-4086出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c00002
关键词
-
资金
- National Natural Science Foundation of China [22074042]
A dual-functional K+ ion-selective electrode was developed for in situ monitoring of extracellular K+ in breast cancer cell lines, showing different responses to electrical stimulation or pH changes. The study provides insights into the microenvironment influence on K+ outflow and the possible mechanism of potassium channels.
The expression of potassium channels can be related to the occurrence and development of tumors. Their change would affect K+ outflow. Thus, in situ monitoring of extracellular K+ shows a great significance. Herein, the dual-functional K+ ion-selective electrode as the scanning electrochemical microscopy (SECM) tip (K+-ISE SECM tip) has been developed for in situ monitoring of the extracellular K+. Based on multi-wall carbon nanotubes as a transduction layer, the K+-ISE SECM tip realizes both the plotting of approach curves to position the tip for in situ detection and the recording of potential responses. It shows a near Nernstian response, good selectivity, and excellent stability. Based on these characteristics, it was used to in situ monitor K+ concentrations ([K+](o)) of three breast cancer cell lines (MCF-7, MDA-MB-231, and SK-BR-3 cells) at 3 mu m above the cell, and [K+](o) of MDA-MB-231 cells show the highest value, followed by MCF-7 cells and SK-BR-3 cells. K+ outflow induced by electrical stimulation or pH changes of the culture environment (Delta[K+](o)) was further determined, and the possible mechanism of K+ outflow was investigated with 4-aminopyridin (4-AP). MCF-7 cells present the largest value of Delta[K+](o), followed by MDA-MB-231 cells and SK-BR-3 cells at all the stimulation potentials, and pH 6.50 shows the greatest impact on K+ outflow of the three cell lines. The pretreatment of 4-AP changed K+ outflow, probably due to the regulation of voltage-gated channels. These findings provide insight into a deep understanding of the microenvironment influence on K+ outflow, thereby reflecting the possible mechanism of potassium channels.
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