期刊
ANALYTICAL CHEMISTRY
卷 94, 期 10, 页码 4352-4358出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c05104
关键词
-
资金
- National Key Research Program of China [2018YFC1602900, 2019YFA0905800]
- National Natural Science Foundation of China [NSFC 21922404]
- Science and Technology Project of Hunan Province [2018JJ3034, 2019SK2201, 2018RS3035, 2020SK3008]
This study developed a new nanoplatform using streptavidin as a carrier to directly analyze and manipulate the activity of target proteins. By incorporating a photoresponsive module, the nanoplatform was able to control the nuclear translocation behavior of active proteins and regulate downstream signaling events.
Directly analyzing and precisely manipulating the activity of target proteins without altering their natural structure and expression would be essential to decoding many protein-dominant cellular processes. To meet this goal, we used streptavidin as the carrier to develop an aptamer-based nanoplatform for monitoring the activation process of specific proteins in living cells. Our results showed that this nanoplatform could efficiently enter the cellular cytoplasm and specifically report the presence of RelA in the activated state. Meanwhile, with incorporation of a photoresponsive module, this aptamer-based nanoplatform was able to manipulate the nuclear translocation behavior of active RelA, enabling control over related downstream signaling events.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据