期刊
ANALYTICAL BIOCHEMISTRY
卷 642, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2022.114562
关键词
Resorcinol; Fluorescence detection; Azamonardine; High selectivity
资金
- National Natural Sci-ence Foundation of China [21505162, 31571874]
- Natural Science Foundation of Hunan Province [2019JJ40167]
- Grain-Oil Process and Quality Control 2011 Collaborative and Innovative Grant from Hunan province, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning [2020KFJJ02]
- Training Program for Excellent Young Innovators of Changsha [kq1802043]
- China Scholarship Council
The proposed fluorescent assay for detecting resorcinol based on its specific chemical reaction with dopamine is sensitive, selective, and rapid, showing great potential for point-of-care testing. The assay demonstrated high sensitivity, anti-interference capability, and accurate quantification of resorcinol contents in various samples, making it promising for practical applications.
We propose a sensitive, selective, and rapid fluorescent assay for detecting resorcinol (RC) based on its specific chemical reaction with dopamine. Under alkaline condition, RC would react with dopamine to yield fluorescent azamonardine, which emits strong blue fluorescence and has a superior excitation wavelength at 416 nm and an emission wavelength at 461 nm. The azamonardine with a molecular weight of 258.1 confirmed by ICP-MS has a quantum yield of 71.3%. The reaction is completed within 1 min showing great potential for point-of-care testing. This assay showed high sensitivity and had a good relationship between fluorescent intensity at 461 nm and RC concentration (I-461 = 106.4 + 93.6*C-RC; R-2 = 0.9904) over the range of 0-40 mu M. More importantly, the assay showed a prominent anti-interference from various substances and even can distinguish RC from its isomers, o-dihydroxybenzene and p-dihydroxybenzene. Finally, our assay successfully quantified RC contents in wheat powder and hair dyes with high accuracy.
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