期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 414, 期 13, 页码 3755-3763出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-022-04018-2
关键词
G-quadruplex; Iridium(III) complex; Capture probe; Target dsDNA; Electrochemiluminescence assay
资金
- Natural Science Foundation of Xuzhou City [KC18140]
- Hong Kong Baptist University [FRG2/17-18/003]
- Health and Medical Research Fund [HMRF/14150561]
- National Natural Science Foundation of China [21305053, 21575121, 21775131]
- Hong Kong Baptist University Century Club Sponsorship Scheme 2019
- Interdisciplinary Research Matching Scheme [RC-IRMS/16-17/03]
- Interdisciplinary Research Clusters Matching Scheme [RC-IRCs/17-18/03]
- Collaborative Research Fund [C5026-16G]
- SKLEBA
- HKBU Strategic Development Fund [SKLP_1920_P02]
In this work, an iridium(III) complex was synthesized and its selective interaction with a specific G-quadruplex DNA sequence was studied. Results showed that the complex exhibits high selectivity and can be used as an efficient electrochemiluminescence probe for the detection of double-stranded DNA.
In this work, we synthesized an iridium(III) complex and studied its selective ability to interact with a specific G-quadruplex DNA sequence (GTGGGTAGGGCGGGTTGG). Results showed that the iridium(III) complex exhibits high selectivity for the G-quadruplex DNA and could be used as an efficient electrochemiluminescence (ECL) probe in a switch-on assay format for the detection of double-stranded DNA (dsDNA). To construct the assay, a hairpin-structured capture probe (CP) which was modified by thiol at its 3 ' end and contained the G-quadruplex sequence at its 5 ' end was firstly immobilized on a gold electrode. Upon the specific recognition of the dsDNA sequence with the corresponding CP, the hairpin structure of the CP was opened to free G-quadruplex sequence, forming the G-quadruplex structure with the assistance of K+. Then, the iridium(III) complex was able to specifically interact with the G-quadruplex to produce an obvious ECL signal that was proportional to the dsDNA concentration. Notably, this iridium(III) complex/G-quadruplex-based strategy was universal and was not limited to the analysis of DNA using specific sequences, thus opening a new avenue for the application of the G-quadruplex-selective iridium(III) complex in the field of ECL.
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