Quick and ultra-sensitive digital assay of influenza virus using sub-picoliter microwells

Quick and sensitive virus detection methods have been developed using sub-picoliter microwells. One method uses an aggregation-induced emission (AIE) reagent, and the other uses an enzymatic reaction supported with immunomagnetic beads at a high concentration of 10(8) particles/mL. Examination of influenza A virus detection using the AIE reagent exhibited a detection limit of 3 x 10(5) copies/mL, which was achievable within 1 min of the total measurement time. The high-concentration immunomagnetic beads method exhibited a detection limit as low as 1 x 10(2) copies/mL. The developed methods are effective and practical tools for ultrafast and ultrasensitive virus detection.

Automated summary

Quick and sensitive virus detection methods have been developed using sub-picoliter microwells. The methods include the use of an aggregation-induced emission (AIE) reagent and enzymatic reaction supported with high-concentration immunomagnetic beads. The AIE reagent method achieved a detection limit of 3 x 10^5 copies/mL within 1 minute, while the high-concentration immunomagnetic beads method had a detection limit as low as 1 x 10^2 copies/mL.