期刊
ANALYTICA CHIMICA ACTA
卷 1210, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aca.2022.339883
关键词
Electrochemiluminescence; Immunosensor; Polyoxometalate nanocluster; Luminol; Neuron-specific enolase; Immunoassay
资金
- National Natural Science Foundation of China [21827812, 21890741]
A quenching-typed electrochemiluminescence immunosensing method was proposed for protein detection using tungsten-based polyoxometalate nanoclusters and free radicals. The method demonstrated excellent detection limit and detectable range.
A quenching-typed electrochemiluminescence (ECL) immunosensing method was proposed for protein detection by the redox reaction of tungsten-based polyoxometalate nanoclusters (W-POM NCs) with free radical (O-2(.-)) as the intermediate of co-reactant H2O2. The immunosensor was constructed by co-immobilizing luminol loaded CeVO4/Au nanohybrids and capture antibody on an indium tin oxide electrode, which showed strong ECL emission in the presence of H2O2 due to the catalysis of CeVO4/Au toward its electro-oxidation to produce O-2(.-) via the reversible conversion between Ce3+ and Ce4+. The W-POM NCs were encapsulated in the uniformly sized amino-silica nanosphere (SiO2) to act as a label (W-POM@SiO2) of the secondary antibody. Upon the sandwich -typed immunoreactions with the target, the W-POM NCs were introduced onto the immunosensor surface to reduce O-2(.-), and thus quenched the ECL emission of luminol-H2O2 system. Using neuron-specific enolase (NSE) as a target, the proposed method showed a detection limit of 0.19 pg/mL and detectable range of 0.5 pg/mL to 18 ng/mL. The excellent performance of the proposed ON-OFF strategy demonstrated that the W-based quenching offers an effective tag for immunoassay of protein biomarkers.
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