期刊
ADVANCED FUNCTIONAL MATERIALS
卷 32, 期 37, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.202201541
关键词
assays; diagnostics; microRNA; miR20a; poly(sodium 4-styrene sulfonate)
类别
资金
- European Union [794410]
- Universitat de Valencia
- COST Action [CM1403]
- Brain Pool program -Ministry of Science and ICT through the National Research Foundation of Korea [2021H1D3A2A0204958912]
- French National research agency [ANR-20-CE09-0021-02]
- Seoul National University
- Universite Paris-Saclay
- Universite de Rouen Normandie
- Normandie Universite
- INSA Rouen
- CNRS
- European Regional Development Fund
- Labex SynOrg [ANR-11-LABX-0029]
- Carnot Institute I2C, XL-Chem graduate school [ANR-18-EURE-0020 XL CHEM]
- Region Normandie, MICINN [D2020-115710GB-I00/AEI/10.13039/501100011033]
- Generalitat Valenciana [PROMETEO/2019/080]
- Marie Curie Actions (MSCA) [794410] Funding Source: Marie Curie Actions (MSCA)
This study presents a simple concept of labeling DNA to a thin polymer layer on UCNPs, demonstrating the fully functional preservation of both UCNPs and DNA. The UCNP-DNA nanohybrids show significant potential for advanced DNA-based biosensing and bioimaging.
Despite the significant advancements of developing upconversion nanoparticles (UCNPs) for high performance biosensing and bioimaging, the development of DNA-functionalized UCNPs with thin coatings, efficient surface passivation, and fully functional DNAs for hybridization sensing in biological media remains extremely challenging. Here, a straightforward concept of labeling DNA to a thin polysulfonate polymer layer on UCNPs is presented. Both UCNPs and DNA preserve their full functionality as demonstrated by Forster resonance energy transfer (FRET) hybridization assays with a Cy3-conjugated complementary DNA. The important bioanalytical benefits of the UCNP-DNA nanohybrids are demonstrated by their implementation into rapid and wash-free microRNA hybridization FRET assays. Using a simple fiber-coupled USB-spectrometer and 980 nm diode laser excitation in a 96-well microplate, ratiometric FRET from UCNP-to-Cy3 can quantify miR20a in a 0.01-10 x 10(-9) m concentration range with a detection limit of 30 x 10(-12) m (4.5 fmol of miR20a), which is more than 20-fold lower compared to quantum dot-based FRET assays. The functional thin-coating DNA-UCNP nanohybrids have a strong potential for translating UCNPs into advanced DNA-based biosensing, bioimaging, and high-throughput and point-of-care clinical diagnostics.
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