A Novel Electrochemiluminescence Janus Emitter for Dual-Mode Biosensing

A novel electrochemiluminescence (ECL) Janus emitter realizes dual-mode biosensing for multiple disease-related biomarkers from cells. Each Janus emitter consists of ruthenium (II) complex and luminol polymer doped hemispheres that are spatially separated but function simultaneously, generating intense dual-ECL emissions. This design effectively eliminates optical and electrochemical interferences between different ECL mono-luminophores in comparison with the homogenous nanostructures, thereby improving ECL efficiency and detection sensitivity. Accordingly, the ECL Janus emitters provide a proof of concept for the construction of the ratiometric ECL sensing of H2O2. Furthermore, this Janus emitter enables simultaneous identification and quantification of the intracellular cancer-related dual microRNAs. This work not only inspires the development of ECL Janus emitters, but also highlights their potential in bioanalysis and clinical diagnosis in the future.

Automated summary

A novel electrochemiluminescence (ECL) Janus emitter combining ruthenium (II) complex and luminol polymer doped hemispheres enables dual-mode biosensing for multiple disease-related biomarkers from cells. It eliminates interference between different ECL mono-luminophores, improving ECL efficiency and detection sensitivity. It also enables ratiometric ECL sensing of H2O2 and simultaneous identification and quantification of cancer-related dual microRNAs.