期刊
ACTA BIOMATERIALIA
卷 140, 期 -, 页码 364-378出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2021.11.030
关键词
CAVD; Aortic heart valve; Valvular interstitial cell; Cryogenic electrospinning; Cell culture
资金
- Federal Ministry of Education and Research - BMBF [G000900A/0]
- Friedrich Naumann Foundation
This study developed a 3D in vitro model of the aortic heart valve that resembles the morphology and behavior of the native valve. It used a bi-layered electrospun scaffold to allow the growth of valvular endothelial cells and valvular interstitial cells, and successfully performed calcification studies.
Calcified aortic valve disease (CAVD) is the most prevalent valve disease in the elderly. Targeted pharmacological therapies are limited since the underlying mechanisms of CAVD are not well understood. Appropriate 3D in vitro models could potentially improve our knowledge of the disease. Here, we developed a 3D in vitro aortic heart valve model that resembles the morphology of the valvular extracellular matrix and mimics the mechanical and physiological behavior of the native aortic valve fibrosa and spongiosa. We employed cryogenic electrospinning to engineer a bi-layered cryogenic electrospun scaffold (BCES) with defined morphologies that allowed valvular endothelial cell (VEC) adherence and valvular interstitial cell (VIC) ingrowth into the scaffold. Using a self-designed cell culture insert allowed us to establish the valvular co-culture simultaneously by seeding VICs on one side and VECs on the other side of the electrospun scaffold. Proof-of-principle calcification studies were successfully performed using an established osteogenic culture protocol and the here designed 3D in vitro aortic heart valve model.
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