4.5 Article

Four temporin-derived peptides exhibit antimicrobial and antibiofilm activities against methicillin-resistant Staphylococcus aureus

期刊

ACTA BIOCHIMICA ET BIOPHYSICA SINICA
卷 54, 期 3, 页码 350-360

出版社

SCIENCE PRESS
DOI: 10.3724/abbs.2022013

关键词

antimicrobial peptide; antibacterial activity; antibiofilm activity; methicillin-resistant Staphylococcus aureus

资金

  1. National Natural Science Foundation of China [32060130, 31560593]
  2. Education Department of Hainan Province [Hnjg2021-26]

向作者/读者索取更多资源

Peptides derived from Temporin-GHa cloned from Hylarana guentheri show potent antimicrobial activities against methicillin-resistant Staphylococcus aureus (MRSA) by damaging the bacterial membrane and inhibiting biofilm formation, with low toxicity to human erythrocytes.
Temporin-GHa (GHa) was cloned from Hylarana guentheri, showing a weak antimicrobial activity. In order to improve its bactericidal efficacy, GHaR6R, GHaR7R, GHaR8R and GHaR9W were designed and synthesized. Compared to the parent peptide, the GHa-derived peptides show potent antimicrobial activities against methicillin-resistant Staphylococcus aureus (MRSA), which is the main pathogen with high morbidity and mortality that causes various infections in humans. These peptides exert bactericidal actions on MRSA by permeabilizing the cytoplasmic membranes and damaging membrane integrity. All of the four peptides exhibit excellent stability under harsh conditions, including extreme temperature and salts. Furthermore, they inhibit the formation of biofilm and eradicate mature biofilm of MRSA. The GHa-derived peptides decrease bacterial surface hydrophobicity, auto-aggregation and polysaccharide intercellular adhesion synthesis in concentration-dependent manner. Real-time quantitative reverse transcription PCR analysis revealed that the peptides downregulate the expression of adhesion genes icaADBC involved in biofilm formation. Except for GHaR7R, the other three peptides have low hemolytic toxicity against human erythrocytes. In the presence of human erythrocytes, GHaR7R, GHaR8R and GHaR9W interact with MRSA preferentially. GHaR6R, GHaR8R and GHaR9W show less toxicity toward normal cells HL-7702 and hFOB1.19. These results suggest that the GHa-derived peptides may be promising antimicrobial candidates against MRSA infections.

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