期刊
CCS CHEMISTRY
卷 3, 期 11, 页码 2725-2739出版社
CHINESE CHEMICAL SOC
DOI: 10.31635/ccschem.020.202000479
关键词
fluorescence imaging; G-quadruplex RNA; intranuclear viscosity; nucleoli
资金
- CRI project [2018R1A3B1052702]
- National Research Foundation of Korea (NRF) [201907030009]
- Interne Fondsen KU Leuven/Internal Funds KU Leuven [STG/19/029]
The nucleolus was identified as the main intracellular target of a new fluorescent probe, V-P1, which demonstrated selectivity to RNA G-quadruplexes and sensitivity to viscosity. The probe was able to accurately detect changes in intracellular viscosity under various conditions.
The nucleolus, the locus of ribosome biogenesis, was found to be the predominant intracellular target of a new fluorescent probe, V-P1. In solution, the probe demonstrated both a selectivity to RNA G-quadruplexes and a sensitivity to the viscosity, while G-quadruplex binding did not disturb the viscosity sensing. In cells, confocal and fluorescence lifetime imaging, combined with digestion and competition experiments, lent support to the hypothesis of an RNA-based G-quadruplex as the intracellular target, postulated to be nucleolar ribosomal RNA (rRNA). The probe demonstrated a high sensitivity to viscosity in both the cytoplasm and the nuclear compartment and was used to precisely interrogate the viscosity changes resulting from diverse stimuli, such as temperature, monensin treatment, and etoposide-induced apoptosis. Owing to the putative rRNA G-quadruplex binding in vitro and in vivo, and further combined with a relatively low degree of toxicity, the dye enabled the interrogation of cytoplasm and intranuclear viscosity changes under diverse conditions and found applications in studying the influence and significance of cytoplasm and intranuclear viscosity as well as in gaining insight into the native secondary structure of rRNA in nucleoli. [GRAPHICS] .
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据