Paenibacillus sinopodophylli sp nov., a siderophore-producing endophytic bacterium isolated from roots of Sinopodophyllum hexandrum (Royle) Ying

A Gram-stain-positive, strictly aerobic, rod-shaped, motile and endospore-forming bacterial strain, designated TEGR-3(T), was isolated from the roots of Sinopodophyllum hexandrum collected from the Qinling Mountains in Shaanxi Province, China. Strain TEGR-3(T) produced siderophores and hydrolysed aesculin, starch and CM-cellulose. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TEGR-3T was a member of the genus Paenibacillus, exhibiting the highest sequence similarity to Paenibacillus endophyticus LMG 27297(T) (973%) and Paenibacillus castaneae DSM 19417(T) (97.3%). MK-7 was the only menaquinone detected and anteiso-C-15:0 and C-16:0 were the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and an unidentified lipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The DNA G+C content was 45.2 mol%. DNA DNA relatedness values for strain TEGR-3(T) with respect to its closest phylogenetic relatives Paenibacillus endophyticus LMG 27297(T) and Paenibacillus castaneae DSM 19417(T) were lower than 40%. Based on the phenotypic, phylogenetic and genotypic data, strain TEGR-3(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus sinopodophylli sp. nov. is proposed. The type strain is TEGR-3(T) (=CCTCC AB 2016047(T)=KCTC 33807(T)).