Pedobacter vanadiisoli sp nova, isolated from soil of a vanadium mine

A Gram-stain-negative, light pink, non-motile, rod-shaped, aerobic bacterium, designated strain XNV015(T), was isolated from soil of a vanadium mine. Phylogenetic analyses based on 16S rRNA gene sequences indicated that it belongs to the genus Pedobacter and was closely related to Pedobacter suwonensis DSM 18130(T) (96.93% sequence similarity), Pedobacter alluvionis NWER-II11(T) (96.66 %), Pedobacter terrae DS-57(T) (96.54 %), Pedobacter kyungheensis KACC 16221(T) (96.54%) and Pedobacter soli KACC 14939(T) (96.47%). This strain clearly differed from the closely related species in terms of acid production from rhamnose and ethanol. Menaquinone-7 was the predominant respiratory quinone. The predominant fatty acids included iso-C-15:0, C-16:1 omega 5c, summed feature 3, iso-C-17:0 3-OH and C-17:0 2-OH. The major polar lipids were phosphatidylethanolannine, glycolipids, lipids and aminolipids. The genomic DNA G+C content was 43.8 mol%. The genotypic analysis, biochemical properties, and phenotypic and chemotaxonomic characteristics indicate that strain XNV015(T) represents a novel species of the genus Pedobacter, for which the name Pedobacter vanadiisoli sp. nov. is proposed. The type strain is XNV015(T) (=CCTCC AB 2015319(T)=KCTC 42866(T)).