Bacterioplanoides pacificum gen. nov., sp nov., isolated from seawater of South Pacific Gyre

A Gram-staining-negative, strictly aerobic, transparent and smooth, curved-rod-shaped motile bacterium with a single polar flagellum, designated strain sw100(T), was isolated from surface seawater of South Pacific Gyre during Integrated Ocean Drilling Program Expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain sw100(T) was most closely related to the member of the family Oceanospirillaceae and was distinct from the most closely related genera Bacterioplanes, Thalassolituus and Oceanobacter (95.8, 94.5-95.3 and 94.4% 16S rRNA gene sequence similarities, respectively). Growth occurred in the presence of 0-11% NaCl (w/v, optimum 7%), at 10-37 degrees C (optimum 28 degrees C) and pH 6.0-9.0 (optimum pH 7.0). The major fatty acids (>10% of total fatty acids) are C-16:0, C-16:0 N alcohol and summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7C). The major polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified polar lipids. The DNA G+C content of strain sw100(T) was 55.3 mol%. The major respiratory quinone was ubiquinone-10 (Q-10). On the basis of polyphasic analyses, strain sw100(T) is considered to represent a novel species in a novel genus of the family Oceanospirillaceae, for which the name Bacterioplanoides pacificum gen. nov., sp. nov. is proposed. The type strain is sw100(T) (=JCM 30597(T)=MCCC 1K00501(T)=KCTC 42424(T)).