Flavobacterium tistrianum sp nov., a gliding bacterium isolated from soil

A novel gliding bacterial strain, GB 56.1(T), was obtained from soil at the Sakaerat Biosphere Reserve, in Nakhon Ratchasima province, Thailand; the strain was characterized using a polyphasic approach. Cells were Gram-stain-negative, yellow, rod shaped and devoid of flagella, but showed gliding motility. Phylogenetic analysis based on 16S rRNA gene sequences found that GB 56.1(T) was a member of the genus Flavobacterium and that the strain shared the highest sequence similarities with Flavobacterium nitrogenifigens (98.4%), Flavobacterium anhuiense (98.3 %) and Flavobacterium ginsenosidimutans (97.9%). The similarities of the sequences of all other species of the genus Flavobacterium were below 97.4%. The major respiratory quinone of strain GB 56.1(T) was MK-6; fatty acids were iso-C-15:0, C-16:1 omega 6c/C-16:1 omega 7c, C-16:0 and C-16:0 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylserine, an unidentified amino lipid and four polar lipids. The DNA G+C content of this strain was 34.2 mol%. The DNA-DNA relatedness of GB 56.1(T) was highest against F. anhuiense, with a value of 37.6%. On the basis of morphological, physiological and chemotaxonomic characteristics, DNA-DNA hybridization relatedness and 16S rRNA gene sequence analysis, we conclude that strain GB 56.1(T) represents a novel species, for which the name Flavobacterium tistrianum sp. nov. is proposed. The type strain is GB 56.1(T) (=TISTR 1612(T) =KCTC 42679(T)).