Uliginosibacterium paludis sp nova, isolated from a marsh

A novel bacterial strain, designated KBP-13(T), was isolated from a water sample taken from the Banping Lake Wetland Park in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain KBP-13(T) were Gram -stain -negative, aerobic, poly-beta-hydroxybutyrate-accumulating, motile rods that formed light yellow colonies. Growth occurred at 15-40 C (optimum, 30-40 degrees C), at pH 6.0-8.0 (optimum, pH 6.0) and with 0-2% (w/v) NaCl (optimum, 0%). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KBP-13(T) belonged to the genus Uliginosibacterium within the family Rhodocyclaceae of the class Betaproteobacteria and its most closely related neighbour was Uliginosibacterium gangwonense 5YN10-9(T) with sequence similarity of 96.0 %. Strain KBP-13(T) contained summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6C), C-16:0 and C-14:0 as predominant fatty acids. The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 65.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one uncharacterized aminophospholipid, one uncharacterized aminolipid, two uncharacterized phospholipids and three uncharacterized glycolipids. On the basis of the genotypic, chennotaxonomic and phenotypic data, strain KBP-13(T) represents a novel species in the genus Uliginosibacterium, for which the name Uliginosibacterium paludis sp. nov. is proposed. The type strain is KBP-13(T) (=BCRC 80903(T)=LMG 28837(T)=KCTC 42655(T)).